{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1327294800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39503.tif","Size":46600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39503.jpg","Size":8824044,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39503.zip","Size":46540238,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"free_text":"myofibril contraction"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"}],"PROCESSINGHISTORY":[{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"PARAMETERIMAGED":[{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"elevation","onto_id":"FBbi:00000320"}],"IMAGEDESCRIPTION":{"free_text":"Freeze fracture of Vorticella microstoma. This cell had been placed in a relaxing medium of saturated sodium pyrophosphate plus 0.01M EDTA (Stain Tech. 47:37, 1972) for 3 hours. A myoneme enclosed by ER membrane is fractured at the cell\u2019s periphery. In this cell it appears that the ER wraps around the myoneme. The P-fracture face of the plasma membrane is studded with many IMPs of significant size in contrast to the E-face of the outer alveolar membrane which has few IMPs. Bits of the P-face of the inner alveolar membrane \nshow many IMPs on that face also. The P-face of the cytosolic-touching membrane of the ER and the E-face of the myonemal-touching membrane of the ER are visible. Linear arrays of IMPs on the E-face next to the myoneme may be transmembrane proteins associated with the linkage complex. Mitochondria are the most prominent organelles in the cytosol and their tubular cristae sometimes show signs of decoration with F1F0 ATP synthases. large arrow, direction of shadowing of freeze fracture replica. TEM taken on 11\/11\/75 by R. Allen with JEM 100B operating at 80kV. Neg. 11,015X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"myofibril","onto_id":"GO:0030016"},{"onto_name":"endoplasmic reticulum","onto_id":"GO:0005783"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"}],"RELATIONTOINTACTCELL":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"}],"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"10481","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"DIMENSION":[{"Space":{"Image_size":4420,"Pixel_size":{"unit":"nanometers","value":1.4},"axis":"X"}},{"Space":{"Image_size":5264,"Pixel_size":{"unit":"nanometers","value":1.4},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Vorticella microstoma","onto_id":"NCBITaxon:77619"},"PREPARATION":{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},"VISUALIZATIONMETHODS":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2012) CIL:39503, Vorticella microstoma, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39503","DOI":"doi:10.7295\/W9CIL39503"}}}