Microscopy product ID: 13
2D Image
Description | Zero tilt image from a single tilt series through a 2 um thick section of a Purkinje cell dendrite that has been intracellularly injected and photooxidized |
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Description | This file contains the original tilt images; pccor10.????.f.Z; compressed.To uncompress use the uncompress command in Unix; Also contains the aligned and cropped tilt images; pccor10.????.crop.The file containing the fiducial marks for the alignment |
Animation
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Description | Animation through the aligned tilt series |
Segmentation
Description | manual segmentation using Xvoxtrace; surfaced with Synu using Nuage algorithm; tarfile contains all synu objects listed; the associated Viewdata file required by Synu for viewing; and Xvoxtrace file (pccor10mm.trace) used to contour the objects. |
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Description | Tar file containing original trace file (MM.trace) with manual contours produced with Xvoxtrace and the surface renderings of the dendritic shaft (shaft.synu) and the individual dendritic spines (sp*.synu) in Synu format, along with the Viewdata file required for viewing with Synuview. |
Reconstruction
Description | Tomographic reconstruction of stained Purkinje cell dendrite from rat cerebellum. Maximum intensity projection through the volume, with constrast reversed so that dendrite appears bright against background |
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Description | Pccor10_inv is an unprocessed volume with the contrast reversed (spiny dendrite appears bright) |
Full metadata
- Collection
- Cite This Work
-
Martone, Maryann; Yamada, Naoko; Arbuthnott, Gordun; Ingham, Cali; Young, Stephen J. (2017). Microscopy product ID: 13. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0FX7979
- Creation Date
- Microscopy product: 2000-06-13. Project: 1992-01-01.
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000 IVEM
Strain: Sprague Dawley
- Funding
-
NIH
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resource
- Microscopy product 13 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB13
Other version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22