CIL:40235, Mus musculus, CNS neuron (sensu Vertebrata), glial cell, blood vessel endothelial cell
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- Collection
- Cite This Work
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Ellisman, Mark; Larson, Stephen; Maynard, Sarah; Martone, Maryann; Bushong, Eric (2021). CIL:40235, Mus musculus, CNS neuron (sensu Vertebrata), glial cell, blood vessel endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HH6K68
- Description
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Coronal section of rat brain triple-labeled with fluoromyelin (green), DiI (red), and TO-PRO3 (blue) which stain for myelin, blood vessels, and cell bodies, respectively. A maximum projection image was compiled by acquiring images across the entire rat brain section, and through 16 optical sections spanning the thickness of the section. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. It is also available through the Whole Brain Catalog (http://wholebraincatalog.org).
- Date Issued
- 2021
- Researchers
- Methods
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The animal was perfused with Ringer's solution for 2 minutes followed by DiI for 10 minutes, and then 2% paraformaldehyde for 10 minutes. The brain was post-fixed overnight, and the tissue subsequently sectioned. After buffer washes, sections were stained with Fluoromyelin (1:100 overnight in cold room), rinsed with buffer washes, and subsequently stained TO-PRO3 (1:3000) for 45 minutes at room temp, rinsed and mounted using Gelvatol. 16 z-planes at 5 µm intervals across the entire section used to generate the tiled 3-D mosaic, acquired with an Olympus Fluoview 1000.
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; compartmentalization of stain or label
Visualization methods: DiI; fluorescent labels
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40235
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40235
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J07P8Z5C
Source data
Previous version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12