{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1332302400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40235.tif","Size":700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40235.jpg","Size":58313,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 7294","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=7294"}],"Contributors":["Mark Ellisman"," Stephen Larson","Sarah Maynard","Maryann Martone","Eric Bushong"]},"BIOLOGICALPROCESS":{"onto_name":"central nervous system structural organization","onto_id":"GO:0021597"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Coronal section of rat brain triple-labeled with fluoromyelin (green), DiI (red), and TO-PRO3 (blue) which stain for myelin, blood vessels, and cell bodies, respectively.  A maximum projection image was compiled by acquiring images across the entire rat brain section, and through 16 optical sections spanning the thickness of the section.  This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.  It is also available through the Whole Brain Catalog (http:\/\/wholebraincatalog.org)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"myelin sheath","onto_id":"GO:0043209"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"vibratome-sectioned tissue","onto_id":"FBbi:00000030"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"11116","TECHNICALDETAILS":{"free_text":"The animal was perfused with Ringer's solution for 2 minutes followed by DiI for 10 minutes, and then 2% paraformaldehyde for 10 minutes. The brain was post-fixed overnight, and the tissue subsequently sectioned. After buffer washes, sections were stained with Fluoromyelin (1:100 overnight in cold room), rinsed with buffer washes, and subsequently stained TO-PRO3 (1:3000) for 45 minutes at room temp, rinsed and mounted using Gelvatol.  16 z-planes at 5 µm intervals across the entire section used to generate the tiled 3-D mosaic, acquired with an Olympus Fluoview 1000."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":389,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"DiI","onto_id":"FBbi:00000115"},{"onto_name":"fluorescent labels","onto_id":"FBbi:00000455"}],"CELLTYPE":[{"onto_name":"CNS neuron (sensu Vertebrata)","onto_id":"CL:0000117"},{"onto_name":"glial cell","onto_id":"CL:0000125"},{"onto_name":"blood vessel endothelial cell","onto_id":"CL:0000071"}]}},"Citation":{"Title":"Mark Ellisman,  Stephen Larson, Sarah Maynard, Maryann Martone, Eric Bushong (2012) CIL:40235, Mus musculus, CNS neuron (sensu Vertebrata), glial cell, blood vessel endothelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40235","DOI":"doi:10.7295\/W9CIL40235"}}}