Buchanan, JoAnn (2021). CIL:6230, Danio rerio, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0S75FBR

This study on the zebrafish optic tectum is a series of four images with magnifications ranging from the SEM block face overview to of the zebrafish head to high magnification (80,000X) showing optic tectum synapse.
The first image is a bloc face SEM image at 200x of the epon embedded zebrafish head. The second image is another block face SEM low magnification image of the tectum. The third image is a 60,000X TEM image of a OTO fixation and copper lead en bloc staining of zebrafish optic tectum synapse. This image, the fourth image in the series, is a 80,000X TEM image of a zebrafish optic tectum synapse.

Zebrafish 10 dpf Optic Tectum Synapses. Image collected on a JOEL 1230 at 80 kV using a Gatan 967 slow-scan, cooled CCD camera at 80,000X. 70 nm thick section.

Microwave processing, osmium tetroxoxide/osmid procedure for enhancing membranes, and copper lead en bloc staining.
Procedure for specimen preparation available at http://www.stanford.edu/~redhair/JoAnn_Buchanan.

• 2021

• Buchanan, JoAnn

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; heat fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; lead salt; copper salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2887

• Danio rerio

• CNS neuron (sensu Vertebrata)
• Optic tectum
• Synapse

• Synapse organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 6230

Doi: https://doi.org/10.6075/J0S75FBR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL6230

Reference

• JoAnn Buchanan: https://alleninstitute.org/what-we-do/brain-science/about/team/staff-profiles/joann-buchanan/
• Related resource: http://www.stanford.edu/~redhair/JoAnn_Buchanan/Zebrafish_Optic_Tectum.html

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12