{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1287633600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"6230.tif","Size":3200000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"6230.zip","Size":1052238,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"6230.jpg","Size":393821,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www.stanford.edu\/~redhair\/JoAnn_Buchanan\/Zebrafish_Optic_Tectum.html"}],"Contributors":["Buchanan, JoAnn (Stanford University School of Medicine)"]},"ZEBRAFISH_ANATOMY":[{"onto_name":"optic tectum","onto_id":"ZFA:0000445"}],"BIOLOGICALPROCESS":{"onto_name":"synapse organization","onto_id":"GO:0050808"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"This study on the zebrafish optic tectum is a series of four images with magnifications ranging from the SEM block face overview to of the zebrafish head to high magnification (80,000X) showing optic tectum synapse.\nThe first image is a bloc face SEM image at 200x of the epon embedded zebrafish head.  The second image is another block face SEM low magnification image of the tectum.  The third image is a 60,000X TEM image of a OTO fixation and copper lead en bloc staining of zebrafish optic tectum synapse.  This image, the fourth image in the series, is a 80,000X TEM image of a zebrafish optic tectum synapse.\n\nZebrafish 10 dpf Optic Tectum Synapses. Image collected on a JOEL 1230 at 80 kV using a Gatan 967 slow-scan, cooled CCD camera at 80,000X. 70 nm thick section. \n\nMicrowave processing, osmium tetroxoxide\/osmid procedure for enhancing membranes, and copper lead en bloc staining.\nProcedure for specimen preparation available at http:\/\/www.stanford.edu\/~redhair\/JoAnn_Buchanan."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"synapse","onto_id":"GO:0045202"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"2887","TERMSANDCONDITIONS":{"free_text":"public_domain"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"nanometers","value":1.6},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"nanometers","value":1.6},"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"onto_id":"ZFA:ZFA:0000445"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Danio rerio","onto_id":"NCBITaxon:7955"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"heat fixed tissue","onto_id":"FBbi:00000609"}],"VISUALIZATIONMETHODS":[{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"copper salt","onto_id":"FBbi:00000610"}],"CELLTYPE":{"onto_name":"CNS neuron (sensu Vertebrata)","onto_id":"CL:0000117"}}},"Citation":{"Title":"Buchanan, JoAnn (Stanford University School of Medicine) (2010) CIL:6230, Danio rerio, CNS neuron (sensu Vertebrata). CIL. Dataset","ARK":"ark:\/b7295\/w9cil6230","DOI":"doi:10.7295\/W9CIL6230"}}}