Greb, Christoph; Jacob, Ralf; Schu, Anja (2021). CIL:41313, Canis lupus familiaris, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QN66WX

Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and stained for acetylated tubulin of the primary cilium (red) by immunofluorescence.

• 2021

• Greb, Christoph
• Jacob, Ralf
• Schu, Anja

Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nmImage collected using a True MultiColor Laser TIRF Leica AM TIRF MC. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: total internal reflection microscopy (TIRF)
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope; distribution of a specific protein
Visualization methods: EGFP; primary antibody plus labeled secondary antibody

• Canis lupus familiaris

• Acetylated tubulin
• Epithelial cell
• P-75
• Primary cilium

• MDCK-II

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41313

Doi: https://doi.org/10.6075/J0QN66WX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41313

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2022-08-12