{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1343275200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"41313.tif","Size":1600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"41313.jpg","Size":69965,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Christoph Greb","Ralf Jacob","Anja Schu"]},"CELLLINE":{"onto_name":"MDCK-II","onto_id":"MCC:0000321"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and stained for acetylated tubulin of the primary cilium (red) by immunofluorescence."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"primary cilium","onto_id":"GO:0072372"},{"free_text":"acetylated tubulin"},{"free_text":"P-75"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"}],"TECHNICALDETAILS":{"free_text":"Cells were grown for 5 days prior to imaging with apical TIRF microscopy.  Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nmImage collected using a True MultiColor Laser TIRF Leica AM TIRF MC. See http:\/\/www.leica-microsystems.com\/science-lab\/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy\/ for additional details."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"free_text":"total internal reflection microscopy (TIRF)"},"DIMENSION":[{"Space":{"Image_size":709,"axis":"X"}},{"Space":{"Image_size":708,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Canis lupus familiaris","onto_id":"NCBITaxon:9615"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Christoph Greb, Ralf Jacob, Anja Schu (2012) CIL:41313, Canis lupus familiaris, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41313","DOI":"doi:10.7295\/W9CIL41313"}}}