Fischer, Robert S.; Waterman, Clare M. (2021). CIL:7329, Mus musculus, blood vessel endothelial cell, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06972KH

Primary endothelial cells from transgenic mouse expressing myosin heavy chain 2A fused to mCherry (red), transfected with GFP-myosin (green) regulatory light chain expression vector and demonstrate the role of myosin in cell division. Cells are embedded in 3D collagen gels. mage Name: myosin cell division timelapse
Biological Source: Primary mouse (mus musculus) aortic endothelial cell.
2D images over time. 0.1274 micron/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds. Images collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 400 ms exposure for mCherry and 200 ms for GFP.
See Fischer et al for details regarding preparation. http://www.ncbi.nlm.nih.gov/pubmed/19185493

• 2021

• Fischer, Robert S.
• Waterman, Clare M.

Preparation: unfixed tissue
Relation to intact cell: dispersed cells in vitro; cells in 3D matrix
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data

• Mus musculus

• Blood vessel endothelial cell
• Myosin II complex
• Primary cell line cell

• Actomyosin contractile ring contraction
• Cell division
• Myosin II binding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7329

Doi: https://doi.org/10.6075/J06972KH

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7329

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19185493

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2022-08-12