{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291525200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7329.jpg","Size":76401,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7329.tif","Size":94000000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"7329.zip","Size":93961128,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["NHLBI\/NIH"],"Contributors":["Robert S. Fischer","Clare M. Waterman"],"PUBMED":["19185493"]},"BIOLOGICALPROCESS":[{"onto_name":"cell division","onto_id":"GO:0051301"},{"onto_name":"actomyosin contractile ring contraction","onto_id":"GO:0000916"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Primary endothelial cells from transgenic mouse expressing myosin heavy chain 2A fused to mCherry (red), transfected with GFP-myosin (green) regulatory light chain expression vector and demonstrate the role of myosin in cell division. Cells are embedded in 3D collagen gels. mage Name: myosin cell division timelapse\nBiological Source: Primary mouse (mus musculus) aortic endothelial cell. \n2D images over time.  0.1274 micron\/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds.  Images collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 400 ms exposure for mCherry and 200 ms for GFP.\nSee Fischer et al for details regarding preparation. http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19185493"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"myosin II complex","onto_id":"GO:0016460"},"RELATIONTOINTACTCELL":[{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},{"free_text":"cells in 3D matrix"}],"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"MOLECULARFUNCTION":{"onto_name":"myosin II binding","onto_id":"GO:0045159"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":520,"Pixel_size":{"unit":"microns","value":0.1274},"axis":"X"}},{"Space":{"Image_size":490,"Pixel_size":{"unit":"microns","value":0.1274},"axis":"Y"}},{"Time":{"unit":"seconds","value":20}},{"Wavelength":{"unit":"nanometers","value":"488, 561"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"}],"CELLTYPE":[{"onto_name":"blood vessel endothelial cell","onto_id":"CL:0000071"},{"onto_name":"primary cell line cell","onto_id":"CL:0000001"}]}},"Citation":{"Title":"Robert S. Fischer, Clare M. Waterman (2010) CIL:7329, Mus musculus, blood vessel endothelial cell, primary cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7329","DOI":"doi:10.7295\/W9CIL7329"}}}