Liu, Zhonghua; Zheng, Yixian (2021). CIL:35158, Homo sapiens, cervical epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04B2ZZ1

Live mitotic HeLa cell treated with epsin1 siRNA, DiOC6(3)to label mitotic membranes (green), and Hoechst 33258 to label chromosomes (blue). Confocal images were taken at 0.118 μm steps along the Z-axis. Epsin1 depleted cells show aberrant membrane morphologies compared with control cells (see grouped image).

• 2021

• Liu, Zhonghua
• Zheng, Yixian

A Leica TCS SP5 DM6000B confocal laser scanning microscope with HCX Plan Apo 63x/1.4 oil objective lens was used to capture the images.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Hoechst 33258; DiOC6 (3,3'-dihexyloxacarbocyanine iodide)

• Cell Image Library Group ID: 8461

• Homo sapiens

• Cervical epithelium
• Condensed chromosome
• Mitotic membranes
• Spindle

• HeLa
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35158

Doi: https://doi.org/10.6075/J04B2ZZ1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35158

Reference

• Liu N, Zheng Y, 2009 J. Cell Biol. 186, 473-480 https://www.ncbi.nlm.nih.gov/pubmed/?term=19704018

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12