{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307505600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"35158.tif","Size":338100000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"35158.zip","Size":224148451,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"35158.jpg","Size":313305,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["Liu N, Zheng Y, 2009 J. Cell Biol. 186, 473-480"],"Contributors":["Zhonghua Liu","Yixian Zheng"],"PUBMED":["19704018"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Live mitotic HeLa cell treated with epsin1  siRNA, DiOC6(3)to label mitotic membranes (green), and Hoechst 33258 to label chromosomes (blue). Confocal images were taken at 0.118 μm steps along the Z-axis. Epsin1 depleted cells show aberrant membrane morphologies compared with control cells (see grouped image)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"free_text":"mitotic membranes"},{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"condensed chromosome","onto_id":"GO:0000793"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"8461","TECHNICALDETAILS":{"free_text":"A Leica TCS SP5 DM6000B confocal laser scanning microscope with HCX Plan Apo 63x\/1.4 oil objective lens was used to capture the images."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.03},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.03},"axis":"Y"}},{"Space":{"Image_size":156,"Pixel_size":{"unit":"microns","value":0.118},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"Hoechst 33258","onto_id":"FBbi:00000417"},{"onto_name":"DiOC6 (3,3'-dihexyloxacarbocyanine iodide)","onto_id":"FBbi:00000055"}],"CELLTYPE":{"free_text":"cervical epithelium"}}},"Citation":{"Title":"Zhonghua Liu, Yixian Zheng (2011) CIL:35158, Homo sapiens, cervical epithelium. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35158","DOI":"doi:10.7295\/W9CIL35158"}}}