Hatano, Toshiyuki; Sluder, Greenfield (2021). CIL:25696, Homo sapiens, retinal pigment epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MG7NF6

Time lapse series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using differential interference contrast optics. This time series is grouped in the Cell Image Library with a corresponding movie.

• 2021

• Hatano, Toshiyuki
• Sluder, Greenfield

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica HCX PLAN APO 100x NA 1.35 DIC objective lens. images were captured at 3 second intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8512

• Homo sapiens

• Retinal pigment epithelium

• Cell proliferation
• HTERT-RPE1
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25696

Doi: https://doi.org/10.6075/J0MG7NF6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25696

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12