{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303963200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"25696.jpg","Size":175109,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25696.zip","Size":3374238326,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Toshiyuki Hatano","Greenfield Sluder"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"free_text":"cell proliferation"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"hTERT-RPE1"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Time lapse series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using differential interference contrast optics. This time series is grouped in the Cell Image Library with a corresponding movie."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},"GROUP_ID":"8512","TECHNICALDETAILS":{"free_text":"Cells were cultured on glass coverslips in 10% FBS, DMEM\/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica HCX PLAN APO 100x NA 1.35 DIC objective lens. images were captured at 3 second intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"},"DIMENSION":[{"Space":{"Image_size":1344,"Pixel_size":{"unit":"microns","value":0.064},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.064},"axis":"Y"}},{"Time":{"unit":"seconds","value":3,"frame":"1920"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"retinal pigment epithelium"}}},"Citation":{"Title":"Toshiyuki Hatano, Greenfield Sluder (2011) CIL:25696, Homo sapiens, retinal pigment epithelium. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25696","DOI":"doi:10.7295\/W9CIL25696"}}}