von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15793, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08914KP

16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow. The embryo is treated with 15 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells at time 00:00. Because of a slight asynchrony in mitosis at the time of TSA addition, each macromere exhibits a distinct phenotype. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. The video corresponds to the second segment of video 5 and Fig. S3B in J Cell Biol. 2009 Dec 14;187(6):831-45. Note the control untreated video is the is also part of this image group.

• 2021

• Bement, William M.
• Miller, Ann L.
• Sider, Jenny R.
• Verbrugghe, Koen J.C.
• Von Dassow, George

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6184

• Strongylocentrotus purpuratus

• Aster
• Ensconsin
• Histone
• Microtubule

• Cytokinesis after mitosis
• Embryo
• Mitotic metaphase
• Trichostatin A treatment

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 15793

Doi: https://doi.org/10.6075/J08914KP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL15793

Reference

• J Cell Biol. 2009 Dec 14;187(6):831-45. https://www.ncbi.nlm.nih.gov/pubmed/?term=20008563

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2022-08-12