{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1300507200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"15793.zip","Size":12005504,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"15793.jpg","Size":46164,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"15793_web.mp4","Size":890760,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 2009 Dec 14;187(6):831-45."],"Contributors":["George von Dassow","Koen J.C. Verbrugghe","Ann L. Miller","Jenny R. Sider","William M. Bement"],"PUBMED":["20008563"]},"BIOLOGICALPROCESS":[{"free_text":"trichostatin A treatment"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"cytokinesis after mitosis","onto_id":"GO:0000281"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Embryo","onto_id":"FMA:69068"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow.  The embryo is treated with 15 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells at time 00:00. Because of a slight asynchrony in mitosis at the time of TSA addition, each macromere exhibits a distinct phenotype. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14\u201317°C.  The video corresponds to the second segment of video 5 and Fig. S3B in J Cell Biol. 2009 Dec 14;187(6):831-45.  Note the control untreated video is the is also part of this image group."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"free_text":"ensconsin"},{"onto_name":"aster","onto_id":"GO:0005818"},{"free_text":"histone"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"6184","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":420,"Pixel_size":{"unit":"microns","value":0.26},"axis":"X"}},{"Space":{"Image_size":420,"Pixel_size":{"unit":"microns","value":0.26},"axis":"Y"}},{"Time":{"unit":"seconds","value":12,"frame":"169"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Strongylocentrotus purpuratus","onto_id":"NCBITaxon:7668"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"}]}},"Citation":{"Title":"George von Dassow, Koen J.C. Verbrugghe, Ann L. Miller, Jenny R. Sider, William M. Bement (2011) CIL:15793, Strongylocentrotus purpuratus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil15793","DOI":"doi:10.7295\/W9CIL15793"}}}