Perlstein, Ethan; Korostyshevsky, Daniel (2021). CIL:39709, Saccharomyces cerevisiae, BY4716. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RF5STX

Transmission electron micrograph of a yeast cell treated with 60µM sertraline. This is a high-magnification zoom of CIL 39708 showing a double-membrane autophagosome. A cresecent-shaped lipid droplet is visible, as well as multiple vesicular structures. The image contributor asks Library users to use the comments box to answer the question, what are the electron dense deposits on the surface of the lipid droplet?

• 2021

• Korostyshevsky, Daniel
• Perlstein, Ethan

BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11310

• Saccharomyces cerevisiae

• Autophagic vacuole
• Autophagic vacuole membrane
• BY4716
• Lipid particle
• Vesicle

• Sertraline treatment
• Vacuole organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39709

Doi: https://doi.org/10.6075/J0RF5STX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39709

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2022-08-12