{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329800400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39709.tif","Size":19800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39709.jpg","Size":1852939,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["Princeton EM Core Facility"],"Contributors":["Ethan Perlstein","Daniel Korostyshevsky"]},"BIOLOGICALPROCESS":[{"onto_name":"vacuole organization","onto_id":"GO:0007033"},{"free_text":"sertraline treatement"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of a yeast cell treated with 60µM sertraline.  This is a high-magnification zoom of CIL 39708 showing a double-membrane autophagosome. A cresecent-shaped lipid droplet is visible, as well as multiple vesicular structures.  The image contributor asks Library users to use the comments box to answer the question, what are the electron dense deposits on the surface of the lipid droplet?"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"autophagic vacuole","onto_id":"GO:0005776"},{"onto_name":"autophagic vacuole membrane","onto_id":"GO:0000421"},{"onto_name":"lipid particle","onto_id":"GO:0005811"},{"onto_name":"vesicle","onto_id":"GO:0031982"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"11310","TECHNICALDETAILS":{"free_text":"BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c.  Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X.  All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase.  This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":2848,"axis":"X"}},{"Space":{"Image_size":2311,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},"CELLTYPE":{"free_text":"BY4716"}}},"Citation":{"Title":"Ethan Perlstein, Daniel Korostyshevsky (2012) CIL:39709, Saccharomyces cerevisiae, BY4716. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39709","DOI":"doi:10.7295\/W9CIL39709"}}}