Wheeler, Richard (2021). CIL:10601, Leishmania mexicana, parasite. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0930RXG

Single Leishmania mexicana cell during late mitosis showing the dividing nucleus and kinetoplast (blue DAPI channel) and the sub-pellicular microtubule array and spindle microtubule arrangement (green KMX-1 channel). Promastigote form Leishmania mexicana (WHO strain MNYC/BZ/62/M379) were grown in M199 (10% FCS, pH 7.4, 28°C). Fixed with 2% paraformaldehyde. DNA labelled with 4',6-diamidino-2-phenylindole (DAPI) and tubulin lebelled with KMX-1 anti-beta tubulin monoclonal antibody (Birkett et al. 1985 FEBS Lett. Aug 5;187(2):211-8. PMID: 4018260). Z stack of 35 images with a z spacing of 0.194 um captured with a 100x/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu). Note there is also a phase channel image that is not currently displayed, but can be turned on in the detailed viewer.

• 2021

• Wheeler, Richard

Preparation: formaldehyde fixed tissue
Relation to intact cell: permeabilized tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy; fluorescence microscopy; phase contrast microscopy
Parameter imaged: optical path length gradient; fluorescence emission
Source of contrast: boundaries between regions with different refractive index; distribution of a specific protein
Visualization methods: Fluorescein (FITC); 4',6-diamidino-2-phenylindole (DAPI)
Processing history: formaldehyde fixed tissue
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3667

• Leishmania mexicana

• Microtubule cytoskeleton
• Nucleus
• Parasite

• Promastigote form
• WHO strain MNYC/BZ/62/M379

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10601

Doi: https://doi.org/10.6075/J0930RXG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10601

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

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2022-08-12