{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1293080400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"10601.tif","Size":86200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"10601.jpg","Size":25682,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"10601.zip","Size":86049967,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Wheeler"]},"BIOLOGICALPROCESS":{"free_text":"promastigote form"},"PROCESSINGHISTORY":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"CELLLINE":{"free_text":"WHO strain MNYC\/BZ\/62\/M379"},"PARAMETERIMAGED":[{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"}],"IMAGEDESCRIPTION":{"free_text":"Single Leishmania mexicana cell during late mitosis showing the dividing nucleus and kinetoplast (blue DAPI channel) and the sub-pellicular microtubule array and spindle microtubule arrangement (green KMX-1 channel). Promastigote form Leishmania mexicana (WHO strain MNYC\/BZ\/62\/M379) were grown in M199 (10% FCS, pH 7.4, 28°C). Fixed with 2% paraformaldehyde. DNA labelled with 4',6-diamidino-2-phenylindole (DAPI) and tubulin lebelled with KMX-1 anti-beta tubulin monoclonal antibody (Birkett et al. 1985 FEBS Lett. Aug 5;187(2):211-8. PMID: 4018260). Z stack of 35 images with a z spacing of 0.194 um captured with a 100x\/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu).  Note there is also a phase channel image that is not currently displayed, but can be turned on in the detailed viewer."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},"SOURCEOFCONTRAST":[{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"}],"GROUP_ID":"3667","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"}],"DIMENSION":[{"Space":{"Image_size":640,"Pixel_size":{"unit":"microns","value":0.0641},"axis":"X"}},{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.0641},"axis":"Y"}},{"Space":{"Pixel_size":{"unit":"microns","value":0.194},"axis":"Z"}},{"Wavelength":{"unit":"nanometers","value":"DAPI, FITC"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Leishmania mexicana","onto_id":"NCBITaxon:5665"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"}],"CELLTYPE":{"free_text":"parasite"}}},"Citation":{"Title":"Richard Wheeler (2010) CIL:10601, Leishmania mexicana, parasite. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10601","DOI":"doi:10.7295\/W9CIL10601"}}}