Allen, Richard (2021). CIL:17446, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FX7850

In a cell treated with trifluoperizine (TFP), all stages of endocytosis and movement to the early endosomes and out of the early endosomes can be found. These steps begin at the parasomal sac and ending with an uncoated carrier vesicles ready to pass into the cell's interior. This micrograph is of the oral region. TEM taken on 10/9/87 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. Adapted with permission from Fig. 15 published in J. Cell Sci. 101:449-461, 1992. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12616) is in the library and available for quantitative analysis. Following trifluoperizine (TFP) treatment, standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue; trifluoperizine (TFP)
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt; horseradish peroxidase
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Cell Image Library Group ID: 5821

• Paramecium multimicronucleatum

• Cell by organism
• Cell cortex
• Ciliated Protist
• Clathrin coat of endocytic vesicle
• Cortical cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist

• Clathrin coating of Golgi vesicle, plasma membrane to endosome targeting
• Cortical cytoskeleton organization
• Cytoplasm organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 17446

Doi: https://doi.org/10.6075/J0FX7850

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL17446

Reference

• J. Cell Sci. 101:449-461, 1992 https://www.ncbi.nlm.nih.gov/pubmed/?term=1629255

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2022-08-12