{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1299387600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"17446.zip","Size":11535486,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"17446.jpg","Size":1620470,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"17446.tif","Size":5700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J. Cell Sci. 101:449-461, 1992"],"Contributors":["Richard Allen (University of Hawaii)"],"PUBMED":["1629255"]},"BIOLOGICALPROCESS":[{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"},{"onto_name":"clathrin coating of Golgi vesicle, plasma membrane to endosome targeting","onto_id":"GO:0010785"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"In a cell treated with trifluoperizine (TFP), all stages of endocytosis and movement to the early endosomes and out of the early endosomes can be found. These steps begin at the parasomal sac and ending with an uncoated carrier vesicles ready to pass into the cell\u2019s interior. This micrograph is of the oral region. TEM taken on 10\/9\/87 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. Adapted with permission from Fig. 15 published in J. Cell Sci. 101:449-461, 1992. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12616) is in the library and available for quantitative analysis. Following trifluoperizine (TFP) treatment, standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"cortical cytoskeleton","onto_id":"GO:0030863"},{"onto_name":"clathrin coat of endocytic vesicle","onto_id":"GO:0030128"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"5821","DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2304,"axis":"X"}},{"Space":{"Image_size":2454,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"free_text":"trifluoperizine (TFP)"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"horseradish peroxidase","onto_id":"FBbi:00000138"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:17446, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil17446","DOI":"doi:10.7295\/W9CIL17446"}}}