Satpute-Krishnan, Prasanna (2021). CIL:9969, Rattus, permanent cell line cell, permanent cell line cell, Kidney. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V40TCF

Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. This file is the merged CFP/YFP time series demonstrating the organization of the early secretory pathway (Golgi and intermediate compartment).

Images were collected on a Zeiss Spinning Disk confocal as a single z slice over time with CFP imaged using 445ht and YFP imaged using 515ht and a 63X oil objective. Images were collected every 2 minutes. Exposure time: 515 was 50ms; 445 was 200ms: for both channels, the gain was 3, the camera intensification was 200, the laser neutral density was 10.

• 2021

• Satpute-Krishnan, Prasanna

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EYFP; ECFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 2315

• Rattus

• Endoplasmic reticulum-Golgi intermediate compartment
• Golgi apparatus
• Kidney
• Permanent cell line cell

• Constitutive secretory pathway
• NRK

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9969

Doi: https://doi.org/10.6075/J0V40TCF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9969

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12