{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291093200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"9969.jpg","Size":77972,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"9969.tif","Size":25200000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"9969.zip","Size":25167631,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Prasanna Satpute-Krishnan"]},"BIOLOGICALPROCESS":{"onto_name":"constitutive secretory pathway","onto_id":"GO:0045054"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"NRK"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. This file is the merged CFP\/YFP time series demonstrating the organization of the early secretory pathway (Golgi and intermediate compartment).   \n\nImages were collected on a Zeiss Spinning Disk confocal as a single z slice over time with CFP imaged using 445ht and YFP imaged using 515ht and a 63X oil objective.  Images were collected every 2 minutes.  Exposure time: 515 was 50ms;  445 was 200ms: for both channels, the gain was 3, the camera intensification was 200, the laser neutral density was 10."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"endoplasmic reticulum-Golgi intermediate compartment","onto_id":"GO:0005793"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"2315","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.174},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.174},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"445, 515"}},{"Time":{"unit":"seconds","value":120}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus","onto_id":"NCBITaxon:10114"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EYFP","onto_id":"FBbi:00000084"},{"onto_name":"ECFP","onto_id":"FBbi:00000081"}],"CELLTYPE":[{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"},{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"},{"onto_name":"Kidney","onto_id":"FMA:7203"}]}},"Citation":{"Title":"Prasanna Satpute-Krishnan (2010) CIL:9969, Rattus, permanent cell line cell, permanent cell line cell, Kidney. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9969","DOI":"doi:10.7295\/W9CIL9969"}}}