Hotta, Azusa; Kawakatsu, Tomomi; Nakatani, Tomoya; Sato, Toshitaka; Matsui, Chiyuki; Sukezane, Taiko; Akagi, Tsuyoshi; Hamaji, Tomoki; Grigoriev, Ilya; Akhmanova, Anna; Takai, Yoshimi; Mimori-Kiyosue, Yuko (2021). CIL:12292, Mus musculus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GQ6WGB

Colocalization of the microtubule anchoring factor LL5α (red) with integrin α6 (blue) in the basal cortex of outer epithelium of mouse mammary gland tissue, and laminin (green) in the basement membrane. Overall findings from this publication showed that signaling from laminin-integrin associations play a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file from Fig. 4Bb, J Cell Biol (2010) 189 (5):901-917.

• 2021

• Akagi, Tsuyoshi
• Akhmanova, Anna
• Grigoriev, Ilya
• Hamaji, Tomoki
• Hotta, Azusa
• Kawakatsu, Tomomi
• Matsui, Chiyuki
• Mimori-Kiyosue, Yuko
• Nakatani, Tomoya
• Sato, Toshitaka
• Sukezane, Taiko
• Takai, Yoshimi

Mammary tissues from C57BL6/J strain mice (8 wk of age) were prepared by perfusion fixation, processed as described previously (Nakatani et al., 2004), and immunostained for laminin (Cy2, 488 nm laser), LL5 (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system.

Preparation: formaldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: Cy2; X-Rhodamine; Cy5
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 8194

• Mus musculus

• Basal cortex
• Basement membrane
• Epithelial cell
• Lateral plasma membrane
• Microtubule

• Cell division
• Cell-substrate adhesion
• Epithelial cell migration
• Establishment or maintenance of apical/basal cell polarity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12292

Doi: https://doi.org/10.6075/J0GQ6WGB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12292

Reference

• J. Cell Biol. 2010. 189(5):901-917 https://www.ncbi.nlm.nih.gov/pubmed/?term=0513766

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12