{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305777600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"12292.tif","Size":3200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"12292.jpg","Size":160768,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2852\/5382\/"}],"PUBLISHED":["J. Cell Biol. 2010. 189(5):901-917"],"Contributors":["Azusa Hotta","Tomomi Kawakatsu","Tomoya Nakatani","Toshitaka Sato","Chiyuki Matsui","Taiko Sukezane","Tsuyoshi Akagi","Tomoki Hamaji","Ilya Grigoriev","Anna Akhmanova","Yoshimi Takai","Yuko Mimori-Kiyosue"],"PUBMED":["PMID:20513769"]},"BIOLOGICALPROCESS":[{"onto_name":"cell-substrate adhesion","onto_id":"GO:0031589"},{"onto_name":"epithelial cell migration","onto_id":"GO:0010631"},{"onto_name":"establishment or maintenance of apical\/basal cell polarity","onto_id":"GO:0035088"},{"onto_name":"cell division","onto_id":"GO:0051301"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Colocalization of the microtubule anchoring factor LL5α (red) with integrin α6 (blue) in the basal cortex of outer epithelium of mouse mammary gland tissue, and laminin (green) in the basement membrane.  Overall findings from this publication showed that signaling from laminin-integrin associations play a role in attaching microtubule plus ends to the epithelial basal cell cortex. This image is the original data file from Fig. 4Bb, J Cell Biol (2010) 189 (5):901-917."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"basal cortex","onto_id":"GO:0045180"},{"onto_name":"lateral plasma membrane","onto_id":"GO:0016328"},{"onto_name":"basement membrane","onto_id":"GO:0005604"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"8194","TECHNICALDETAILS":{"free_text":"Mammary tissues from C57BL6\/J strain mice (8 wk of age) were prepared by perfusion fixation, processed as described previously (Nakatani et al., 2004), and immunostained for laminin (Cy2, 488 nm laser), LL5  (rhodamine red-X, 543 nm laser) and integrin a6 (Cy5, 633 nm laser), and observed using a confocal laser-scanning microscope system."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.09},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.09},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Cy2","onto_id":"FBbi:00000448"},{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},{"onto_name":"Cy5","onto_id":"FBbi:00000450"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Azusa Hotta, Tomomi Kawakatsu, Tomoya Nakatani, Toshitaka Sato, Chiyuki Matsui, Taiko Sukezane, Tsuyoshi Akagi, Tomoki Hamaji, Ilya Grigoriev, Anna Akhmanova, Yoshimi Takai, Yuko Mimori-Kiyosue (2011) CIL:12292, Mus musculus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12292","DOI":"doi:10.7295\/W9CIL12292"}}}