Microscopy product ID: 3433
2D Image
Description | Zero degree tilt image through a 0.25 um thick section of blue green algae grown under high light conditions and imaged using intermediate voltage electron microscopy. |
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Description | Zip file containing the full resolution digitized unaligned images (*.f), along with the cropped and aligned images used for reconstruction (*.crop), the file containing the fiducial marks (*.fido) and the list of angles used for reconstruction (*.ang). |
Segmentation
Description | Manual segmentation of the chloroplast membrane and thylakoid membranes using Xvoxtrace 2.1 followed by surfacing with Synu. Synu surfaces were also converted into iv format for viewing with Amira. |
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Description | Zip file containing Xvoxtrace file with manual tracings, surfaced segmented objects and random snapshots of the rendered objects. Multiple Xvoxtrace files are included, one set with the prefix "fixed", presumably reflecting the correction of some of the original tracings. Surfaced objects are included in both Synu (*.synu) and Amira (*.iv) formats, along with the ancillary files required for viewing in these programs (Viewdata for Synu and .hx for Amira). Also included are multiple snapshots of the rendered objects in .ppm format. |
Reconstruction
Description | Single computed slice through a tomographic volume of a blue green algae grown under high light conditions. |
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Description | Zip file containing the tomographic volume in Analyze 7.5 format (phaeo28_obl_sub.img/hdr). |
Animation
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Description | Animation through the computed slices of a tomographic volume of a blue green algae grown under high light conditions. Dark particles are 20 nm gold particles that were placed on the surface of the section to serve as fiducial marks. |
Full metadata
- Collection
- Cite This Work
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Moisan, Tiffany; Sosinsky, Gina; Buitenhuys, Casey; Ellisman, Mark H. (2017). Microscopy product ID: 3433. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0930SZB
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
Strain: Karsten
- Funding
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National Aeronautics and Space Administration
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resources
- Moisan TA, Ellisman MH, Buitenhuys CW, Sosinsky GE (2006). Differences in chloroplast ultrastructure of Phaeocystis antarctica in low and high light. Marine Biology, 149(6):1281-1290. https://doi.org/10.1007/s00227-006-0321-5
- Microscopy product 3433 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3433
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22