Kim, Sungsu; Wairkar, Yogesh P.; Daniels, Richard W.; DiAntonio, Aaron (2021). CIL:13470, Drosophila melanogaster, garland cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HH6HRM

Transmission electron micrograph of Drosophila nephrocyte garland cell. Wild-type garland cells display extensive tubulo-vesicular and vacuolar profiles of endosomal compartments, including electron-lucent alpha vacuoles akin to early endosomes in the cell cortex, and electron-dense beta vacuoles that represent late endosomes more centrally. The largest vacuoles in wild type are 2 microns in diameter and all vacuoles contain a single aggregate of electron-dense granular material that appears to be attached to the limiting membrane. Samples for TEM were fixed for 1 h at 4°C in 2% paraformaldehyde, 2% glutaraldehyde, and 1% tannic acid in 0.1 M cacodylic acid buffer, pH 7.2. Then, the samples were postfixed in 1% OsO4 in 0.1 M cacodylic acid buffer, pH 7.2, for 1 h at room temperature, stained en bloc with 1% uranyl acetate, dehydrated in a grade series of ethanol and propylene oxide, and embedded in epon resin (Electron Microscopy Sciences). Blocks were sectioned in an ultramicrotome (RMC Products) at ∼70-nm thickness with a Delaware Diamond knife and post-stained for 1 h in Reynolds lead citrate and uranyl acetate. Electron micrographs were taken on a transmission electron microscope (H-7500; Hitachi) using an AMT camera system. Mag: 5000x. Image corresponds to Figure 2B, left WT panel in Kim et al. J Cell Biol. 188: 717-734. 2010. A high magnification view of this region is in CIL# 13471.

• 2021

• Daniels, Richard W.
• DiAntonio, Aaron
• Kim, Sungsu
• Wairkar, Yogesh P.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 6022

• Drosophila melanogaster

• Early endosome
• Garland cell
• Late endosome
• Nucleus

• Endocytosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13470

Doi: https://doi.org/10.6075/J0HH6HRM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13470

Reference

• J Cell Biol. 188: 717-734. 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=20194640

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12