{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1300334400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13470.tif","Size":1300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13470.jpg","Size":224738,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13470.zip","Size":1217457,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2252\/"}],"PUBLISHED":["J Cell Biol. 188: 717-734. 2010"],"Contributors":["Sungsu Kim","Yogesh P. Wairkar","Richard W. Daniels","Aaron DiAntonio"],"PUBMED":["PMID: 20194640"]},"BIOLOGICALPROCESS":{"onto_name":"endocytosis","onto_id":"GO:0006897"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of Drosophila nephrocyte garland cell. Wild-type garland cells display extensive tubulo-vesicular and vacuolar profiles of endosomal compartments, including electron-lucent alpha vacuoles akin to early endosomes in the cell cortex, and electron-dense beta vacuoles that represent late endosomes more centrally.  The largest vacuoles in wild type are 2 microns in diameter and all vacuoles contain a single aggregate of electron-dense granular material that appears to be attached to the limiting membrane. Samples for TEM were fixed for 1 h at 4°C in 2% paraformaldehyde, 2% glutaraldehyde, and 1% tannic acid in 0.1 M cacodylic acid buffer, pH 7.2. Then, the samples were postfixed in 1% OsO4 in 0.1 M cacodylic acid buffer, pH 7.2, for 1 h at room temperature, stained en bloc with 1% uranyl acetate, dehydrated in a grade series of ethanol and propylene oxide, and embedded in epon resin (Electron Microscopy Sciences). Blocks were sectioned in an ultramicrotome (RMC Products) at ∼70-nm thickness with a Delaware Diamond knife and post-stained for 1 h in Reynolds lead citrate and uranyl acetate. Electron micrographs were taken on a transmission electron microscope (H-7500; Hitachi) using an AMT camera system. Mag: 5000x.  Image corresponds to Figure 2B, left WT panel in Kim et al.  J Cell Biol. 188: 717-734. 2010. A high magnification view of this region is in CIL# 13471."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"}],"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"early endosome","onto_id":"GO:0005769"},{"onto_name":"late endosome","onto_id":"GO:0005770"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"6022","DATAQUALIFICATION":{"free_text":"RAW;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1024,"axis":"X"}},{"Space":{"Image_size":1184,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":{"onto_name":"garland cell","onto_id":"CL:0000486"}}},"Citation":{"Title":"Sungsu Kim, Yogesh P. Wairkar, Richard W. Daniels, Aaron DiAntonio (2011) CIL:13470, Drosophila melanogaster, garland cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13470","DOI":"doi:10.7295\/W9CIL13470"}}}