CIL:36631, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36631, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MS3RP5
- Description
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Occasionally mitochondria of Paramecium become filled with cristae that display a wavy or helical pattern. In thin sections these cristae are viewed as curved arcs as well as cross sectioned rows in different parts of the organelle. Stacks of decorated tubules of the contractile vacuole system are also seen in this micrograph. Cristae of mitochondria bear F1F0 ATP synthases while the decorated tubules bear the related V1V0 V-ATPase proton pump. The helical arrayed F1F0 and V1V0 units may be responsible for forming the tubular nature of the cristae and the tubular decorated spongiome (for discussion of this hypothesis see Allen, Protoplasma 189:1-8, 1995). TEM taken on 5/6/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,250X. Bar = 0.5µm.
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36631
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36631
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12