{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313726400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"36631.jpg","Size":462395,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"36631.tif","Size":1400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"36631.zip","Size":2721710,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"mitochondrial proton-transporting ATP synthase complex assembly","onto_id":"GO:0033615"},{"onto_name":"vacuolar proton-transporting V-type ATPase complex assembly","onto_id":"GO:0070072"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Occasionally mitochondria of Paramecium become filled with cristae that display a wavy or helical pattern. In thin sections these cristae are viewed as curved arcs as well as cross sectioned rows in different parts of the organelle. Stacks of decorated tubules of the contractile vacuole system are also seen in this micrograph. Cristae of mitochondria bear F1F0 ATP synthases while the decorated tubules bear the related V1V0 V-ATPase proton pump. The helical arrayed F1F0 and V1V0 units may be responsible for forming the tubular nature of the cristae and the tubular decorated spongiome (for discussion of this hypothesis see Allen, Protoplasma 189:1-8, 1995). TEM taken on 5\/6\/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,250X. Bar = 0.5µm."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"contractile vacuole","onto_id":"GO:0000331"},{"onto_name":"integral to contractile vacuolar membrane","onto_id":"GO:0031165"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":1088,"axis":"X"}},{"Space":{"Image_size":1236,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:36631, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36631","DOI":"doi:10.7295\/W9CIL36631"}}}