Garcia, Andres J; Singh, Ankur (2021). CIL:44701, Homo sapiens, fibroblast of lung. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00V8C6B

Immunofluorescence image of human IMR90 lung fibroblasts stained for vinculin (green) and filamentous actin (magenta). Nuclei are stained blue. This image of untreated fibroblasts comes from a study of the changes in adhesion that accompany treatment to induce stem cells and can be used in hiPSC purification. See also Singh et al. 2013 Nat Meth 10:438-444. The image appeared in the May 2013 issue of the NIGMS Biomedical Beat which features noteworthy NIGMS-supported research.

• 2021

• Garcia, Andres J
• Singh, Ankur

IMR90 cells in culture were treated with a cytoskeleton stabilizing buffer, extracted briefly with 0.5% Triton X-100, fixed with 4% paraformaldehyde, and immunostained stained for vinculin using a secondary antibody conjugated with Alexa 488 (green). The actin cytoskeleton was stained with phalloidin conjugated with Alexa 555 (magenta). Nuclei were counterstained with DAPI (blue). Preparations were observed using a Nikon E400 fluorescence microscope with 20x PLANAPO objective lens, and recorded with a SPOT CCD camera.
For further details, see A Singh et al. 2013 Adhesion strength-based, label-free isolation of human pluripotent stem cells. Nat Meth 10:438-444 and associated Supplementary Material.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope
Visualization methods: Alexa Fluor 555; Alexa Fluor 488; 4',6-diamidino-2-phenylindole (DAPI)
Data qualification: Raw;spatialmeasurements

• Homo sapiens

• Cytoskeleton
• Fibroblast of lung

• Cell adhesion
• IMR90

• data
• image

• No linguistic content; Not applicable

Samplenumber: 44701

Doi: https://doi.org/10.6075/J00V8C6B

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL44701

Other resource

• NIGMS Biomedical Beat: https://biobeat.nigms.nih.gov

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2022-08-12