{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1369195200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"44701.jpg","Size":142432,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"44701.tif","Size":1100000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"NIGMS Biomedical Beat","Href":"http:\/\/publications.nigms.nih.gov\/biobeat\/"}],"Contributors":["Andres J Garcia","Ankur Singh"]},"BIOLOGICALPROCESS":{"onto_name":"cell adhesion","onto_id":"GO:0007155"},"CELLLINE":{"free_text":"IMR90"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Immunofluorescence image of human IMR90 lung fibroblasts stained for vinculin (green) and filamentous actin (magenta).  Nuclei are stained blue.  This image of untreated fibroblasts comes from a study of the changes in adhesion that accompany treatment to induce stem cells  and can be used in hiPSC purification. See also Singh et al. 2013 Nat Meth 10:438-444.  The image appeared in the May 2013 issue of the NIGMS Biomedical Beat which features noteworthy NIGMS-supported research."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"TECHNICALDETAILS":{"free_text":"IMR90 cells in culture were treated with a cytoskeleton stabilizing buffer, extracted briefly with 0.5% Triton X-100, fixed with 4% paraformaldehyde, and immunostained stained for vinculin using a secondary antibody conjugated with Alexa 488 (green).  The actin cytoskeleton was stained with phalloidin conjugated with Alexa 555 (magenta). Nuclei were counterstained with DAPI (blue). Preparations were observed using a Nikon E400 fluorescence microscope with 20x PLANAPO objective lens, and recorded with a SPOT CCD camera.\nFor further details, see A Singh et al. 2013 Adhesion strength-based, label-free isolation of human pluripotent stem cells. Nat Meth 10:438-444 and associated Supplementary Material."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":600,"axis":"X"}},{"Space":{"Image_size":600,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 555","onto_id":"FBbi:00000443"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"}],"CELLTYPE":{"onto_name":"fibroblast of lung","onto_id":"CL:0002553"}}},"Citation":{"Title":"Andres J Garcia, Ankur Singh (2013) CIL:44701, Homo sapiens, fibroblast of lung. CIL. Dataset","ARK":"ark:\/b7295\/w9cil44701","DOI":"doi:10.7295\/W9CIL44701"}}}