Vicente-Manzanares, Miguel; Newell-Litwa, Karen; Bachir, Alexia I.; Whitmore, Leanna A.; Horwitz, Alan Rick (2021). CIL:25843, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J67FPG

Time-lapse microscopy of a spreading control Cho-K1 cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin. The arrow points to the site of initial accumulation of RLC-D,D–GFP. Note the protrusion opposite the concentration of RLC-D,D, which results in cell polarization and definition of the rear. Images were captured in TIRF mode using an inverted microscope (IX70; Olympus) coupled to a CCD camera (Retiga Exi; Qimaging). Live samples were acquired at 37°C using CCM1 medium. This movie corresponds to Fig. 1 C. and Video 1 from J Cell Biol 2011 193:381-396.

• 2021

• Bachir, Alexia I.
• Horwitz, Alan Rick
• Newell-Litwa, Karen
• Vicente-Manzanares, Miguel
• Whitmore, Leanna A.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 7724

• Cricetulus griseus

• Epithelial cell
• Myosin regulatory light chain

• CHO-K1
• Phospho-mimetic

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 25843

Doi: https://doi.org/10.6075/J0J67FPG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25843

Reference

• J Cell Biol 2011 193:381-396 https://www.ncbi.nlm.nih.gov/pubmed/?term=21482721

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2022-08-12