{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303963200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"25843_web.mp4","Size":866117,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"25843.jpg","Size":8127,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25843.zip","Size":3244160,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol 2011 193:381-396"],"Contributors":["Miguel Vicente-Manzanares","Karen Newell-Litwa","Alexia I. Bachir","Leanna A. Whitmore","Alan Rick Horwitz"],"PUBMED":["21482721"]},"BIOLOGICALPROCESS":{"free_text":"phospho-mimetic"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"CHO-K1","onto_id":"MCC:0000103"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time-lapse microscopy of a spreading control Cho-K1 cell expressing RLC-D,D\u2013GFP (a phospho-mimetic mutant of the RLC) on fibronectin. The arrow points to the site of initial accumulation of RLC-D,D\u2013GFP. Note the protrusion opposite the concentration of RLC-D,D, which results in cell polarization and definition of the rear.  Images were captured in TIRF mode using an inverted microscope (IX70; Olympus) coupled to a CCD camera (Retiga Exi; Qimaging). Live samples were acquired at 37°C using CCM1 medium.  This movie corresponds to Fig. 1 C. and Video 1 from J Cell Biol 2011 193:381-396."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"myosin regulatory light chain"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7724","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":417,"axis":"X"}},{"Space":{"Image_size":378,"axis":"Y"}},{"Time":{"unit":"seconds","value":5}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25843, Cricetulus griseus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25843","DOI":"doi:10.7295\/W9CIL25843"}}}