Cell Image Library (2021). CIL:39730. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0416VXR

TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were compacted in the appropriate salt, and mildly crosslinked with formaldehyde to preserve nucleosome-nucleosome interactions, then allowed to decondense in low salt prior to fixation with glutaraldehyde, and shadowing with Pt. (B-D with linker histone and without Mg; J-M) with linker histone and 1 mM MgCl2; (N-S)with linker histone and 4mM MgCl2. The drawings at right show the authors' interpretation of the adjacent micrograph showing crosslinked nucleosomes and the number of nucleosomes included in the 'loop' created by the crosslink.
See Fig 3 in S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322
See Fig 1 in: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322

• 2021

Reconstitutes were fixed in the appropriate salt environment, applied to thin carbon films, air dried, and shadowed with Pt using electron bean evaporation (Balzers BA 080 vacuum evaporator. Grids were examined in a FEI Tecnai 12 TEM at 100KV with a LaB6 filament and tilted darkfield optics, and recorded using a TVIPS 2k CCD camera.
See also: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322.

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: CCD
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Data qualification: Processed

• Cell Image Library Group ID: 10719

• Chromatin
• Nucleosome

• Chromatin organization
• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39730

Doi: https://doi.org/10.6075/J0416VXR

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39730

Reference

• S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322 https://www.ncbi.nlm.nih.gov/pubmed/?term=19651606
• Sergei A. Grigoryev, Gaurav Arya, Sarah Correll, Christopher L. Woodcock, and Tamar Schlick (2009). Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. PNAS. https://doi.org/10.1073/pnas.0903280106

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2022-08-12