{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329109200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39730.tif","Size":700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39730.jpg","Size":90162,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39730.zip","Size":624054,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Proceedings National Academy of Sciences USA","Href":"http:\/\/www.pnas.org\/content\/106\/32\/13317.long"}],"PUBLISHED":["S A  Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322"],"PUBMED":["19651606"]},"BIOLOGICALPROCESS":[{"onto_name":"DNA packaging","onto_id":"GO:0006323"},{"onto_name":"chromatin organization","onto_id":"GO:0006325"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were compacted in the appropriate salt, and mildly crosslinked with formaldehyde to preserve nucleosome-nucleosome interactions, then allowed to decondense in low salt prior to fixation with glutaraldehyde, and shadowing with Pt.  (B-D with linker histone and without Mg; J-M) with linker histone and 1 mM MgCl2; (N-S)with linker histone and 4mM MgCl2. The drawings at right show the authors' interpretation of the adjacent micrograph showing crosslinked nucleosomes and the number of nucleosomes included in the 'loop' created by the crosslink.\nSee Fig 3 in S A  Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322 \nSee Fig 1 in: S A  Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322"},"ITEMTYPE":{"free_text":"CCD"},"CELLULARCOMPONENT":[{"free_text":"chromatin"},{"onto_name":"nucleosome","onto_id":"GO:0000786"}],"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"10719","TECHNICALDETAILS":{"free_text":"Reconstitutes were fixed in the appropriate salt environment, applied to thin carbon films, air dried, and shadowed with Pt using electron bean evaporation (Balzers BA 080 vacuum evaporator.  Grids were examined in a FEI Tecnai 12 TEM at 100KV with a LaB6 filament and tilted darkfield optics, and recorded using a TVIPS 2k CCD camera.\nSee also: S A  Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":495,"axis":"X"}},{"Space":{"Image_size":420,"axis":"Y"}}],"PREPARATION":{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"}}},"Citation":{"Title":"CIL (2012) CIL:39730. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39730","DOI":"doi:10.7295\/W9CIL39730"}}}