Cell Image Library (2021). CIL:36022, Chlorocebus aethiops, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0K93664

COS7 cells, transfected with GFP-PTP1B and APN-EphA3, when co-transfected with TM-BirA, yielded biotinylated EphA3 that can be stimulated with SA-Dynabeads. GFP-PTP1B and EphA3-transfected COS7 cells were stimulated with Alexa Fluor 594–ephrinA5–Fc–coated Protein A Dynabeads. Cell surface EphA3 was labelled on intact with anti-EphA3 mAb and detected with Alexa546-labelled secondary antibodies by confocal microscopy.

• 2021

Preparation: chemically fixed tissue
Relation to intact cell: whole mounted tissue
Item type: fluorescence microscopy; bright-field microscopy
Imaging mode: fluorescence microscopy; bright-field microscopy
Parameter imaged: fluorescence emission; absorption of illumination
Source of contrast: distribution of a specific protein; differences in intrinsic optical density
Visualization methods: Green fluorescent proteins from Aequorea; Alexa Fluor 546

• Cell Image Library Group ID: 8847

• Chlorocebus aethiops

• Permanent cell line cell

• COS-7
• Negative regulation of insulin receptor signaling pathway
• Protein tyrosine kinase activity

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36022

Doi: https://doi.org/10.6075/J0K93664

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36022

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=21135139

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2022-08-12