{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1311825600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"36022.zip","Size":6291973,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"36022.tif","Size":6400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36022.jpg","Size":104045,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBMED":["PMID: 21135139"]},"BIOLOGICALPROCESS":{"onto_name":"negative regulation of insulin receptor signaling pathway","onto_id":"GO:0046627"},"CELLLINE":{"onto_name":"COS-7","onto_id":"MCC:0000112"},"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"absorption of illumination","onto_id":"FBbi:00000308"}],"IMAGEDESCRIPTION":{"free_text":"COS7 cells, transfected with GFP-PTP1B and APN-EphA3, when co-transfected with TM-BirA, yielded biotinylated EphA3 that can be stimulated with SA-Dynabeads.  GFP-PTP1B and EphA3-transfected COS7 cells were stimulated with Alexa Fluor 594\u2013ephrinA5\u2013Fc\u2013coated Protein A Dynabeads. Cell surface EphA3 was labelled on intact with anti-EphA3 mAb and detected with Alexa546-labelled secondary antibodies by confocal microscopy."},"ITEMTYPE":[{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"bright-field microscopy","onto_id":"FBbi:00000243"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"differences in intrinsic optical density","onto_id":"FBbi:00000602"}],"GROUP_ID":"8847","MOLECULARFUNCTION":{"onto_name":"protein tyrosine kinase activity","onto_id":"GO:0004713"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"bright-field microscopy","onto_id":"FBbi:00000243"}],"DIMENSION":[{"Space":{"Image_size":1024,"axis":"X"}},{"Space":{"Image_size":1024,"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"onto_id":":1024"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlorocebus aethiops","onto_id":"NCBITaxon:9534"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":[{"onto_name":"Green fluorescent proteins from Aequorea","onto_id":"FBbi:00000437"},{"onto_name":"Alexa Fluor 546","onto_id":"FBbi:00000441"}],"CELLTYPE":{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"},"PATHOLOGICALPROCESS":{"onto_id":":1024"}}},"Citation":{"Title":"CIL (2011) CIL:36022, Chlorocebus aethiops, permanent cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36022","DOI":"doi:10.7295\/W9CIL36022"}}}