Parysek, Linda (2021). CIL:247, Mus musculus, myelinating Schwann cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0057F7B

This ghoulish-looking Schwann cell was present in a primary culture of the dorsal root ganglion of mouse that was transfected with a mutant peripherin transgene. The intermediate filament network (vimentin antibody labeling in green) is collapsed around the nucleus 48 hr after transfection, and is correlated with a corresponding peri-nuclear clustering of the mitochondria (labeled with Mitotracker). Mitotracker CMX Ros was added to the culture medium for 30 mins, then rinsed out for 45 minutes in culture before fixing the cells with methanol.

Microscope - Zeiss Axioplan I
Illumination - Mercury arc lamp
Objective - 100X oil/1.3 NA
Intermediate lens between obj and camera - 0.63X
Camera - Axiocam MRM CCD
Filters:
1. Ex 485/20, Em 515-565 BP, dichroic 510
2. Ex 560/40, Em 630/60, dichroic 595

• 2021

• Parysek, Linda

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: microscopy
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: local accumulation of fluorescent probe
Visualization methods: Alexa Fluor 488; CMX rosamine (Mitotracker Red); primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data

• Mus musculus

• Mitochondrion
• Myelinating Schwann cell
• Type III intermediate filament

• ATP biosynthetic process
• Cytoplasm organization
• Intermediate filament-based process
• Mitochondrion localization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 247

Doi: https://doi.org/10.6075/J0057F7B

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL247

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2022-08-12