{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1285905600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"247.tif","Size":700000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"247.zip","Size":689866,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"247.jpg","Size":31319,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Parysek, Linda"]},"BIOLOGICALPROCESS":[{"onto_name":"intermediate filament-based process","onto_id":"GO:0045103"},{"onto_name":"mitochondrion localization","onto_id":"GO:0051646"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"This ghoulish-looking Schwann cell  was present in a primary culture of the dorsal root ganglion of mouse that was transfected with a mutant peripherin transgene. The intermediate filament network (vimentin antibody labeling in green) is collapsed around the nucleus 48 hr after transfection, and is correlated with a corresponding peri-nuclear clustering of the mitochondria (labeled with Mitotracker).  Mitotracker CMX Ros was added to the culture medium for 30 mins, then rinsed out for 45 minutes in culture before fixing the cells with methanol.\n\nMicroscope - Zeiss Axioplan I\nIllumination - Mercury arc lamp\nObjective - 100X oil\/1.3 NA\nIntermediate lens between obj and camera - 0.63X\nCamera - Axiocam MRM CCD\nFilters:  \n1.  Ex 485\/20, Em 515-565 BP, dichroic 510\n2.  Ex 560\/40, Em 630\/60, dichroic 595"},"ITEMTYPE":{"onto_name":"microscopy","onto_id":"FBbi:00000241"},"CELLULARCOMPONENT":[{"onto_name":"type III intermediate filament","onto_id":"GO:0045098"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"free_text":"local accumulation of fluorescent probe"},"MOLECULARFUNCTION":[{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"ATP biosynthetic process","onto_id":"GO:0006754"}],"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Pixel_size":{"unit":"nanometers","value":104},"axis":"X"}},{"Space":{"Pixel_size":{"unit":"nanometers","value":104},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"CMX rosamine (Mitotracker Red)","onto_id":"FBbi:00000054"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"myelinating Schwann cell","onto_id":"CL:0000218"}}},"Citation":{"Title":"Parysek, Linda (2010) CIL:247, Mus musculus, myelinating Schwann cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil247","DOI":"doi:10.7295\/W9CIL247"}}}