Pasapera, Ana M.; Waterman, Clare M. (2021). CIL:7440, Homo sapiens, primary cell line cell, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CN72WJ

Human umbilical vein endothelial cells (HUVECS) labeled for focal adhesions (green) and F-actin (red). HUVECs were plated on polyacrilamide gels (1.5 Kpa) coated with 1.0 mg/ml fibronectin. After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Phosphotyrosine (4G10) monoclonal antibody detected with goat anti-mouse Alexa 565 and Alexa 488 phalloidin to stain for F-actin.

Image was collected on a Nikon Eclipse with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon). The exposure time was 320 msec each channel. A xenon arc lamp was used with a Semrock DA/FI/TR/Cy5-4x4M-B Sedat filter set.

• 2021

• Pasapera, Ana M.
• Waterman, Clare M.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; phalloidin; primary antibody plus labeled secondary antibody; Alexa Fluor 568
Processing history: unprocessed raw data

• Homo sapiens

• Actin cytoskeleton
• Endothelial cell
• Focal adhesion
• Primary cell line cell

• HUVEC
• Phosphotyrosine binding
• Regulation of focal adhesion assembly

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7440

Doi: https://doi.org/10.6075/J0CN72WJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7440

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=20308429

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2022-08-12