{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290402000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7440.jpg","Size":167499,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7440.tif","Size":4400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"7440.zip","Size":4357598,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["NHLBI\/NIH"],"Contributors":["Ana M. Pasapera","Clare M. Waterman"],"PUBMED":["20308429"]},"BIOLOGICALPROCESS":{"onto_name":"regulation of focal adhesion assembly","onto_id":"GO:0051893"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"HUVEC"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Human umbilical vein endothelial cells (HUVECS) labeled for focal adhesions (green) and F-actin (red).  HUVECs were plated on polyacrilamide gels  (1.5 Kpa) coated with 1.0 mg\/ml fibronectin.  After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Phosphotyrosine (4G10) monoclonal antibody detected with goat anti-mouse Alexa 565 and Alexa 488 phalloidin to stain for F-actin.  \n\nImage was collected on a Nikon Eclipse with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon).  The exposure time was 320 msec each channel. A xenon arc lamp was used with a  Semrock DA\/FI\/TR\/Cy5-4x4M-B Sedat filter set."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"focal adhesion","onto_id":"GO:0005925"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"MOLECULARFUNCTION":{"onto_name":"phosphotyrosine binding","onto_id":"GO:0001784"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1392,"Pixel_size":{"unit":"microns","value":0.107},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.107},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"488, 568"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"phalloidin","onto_id":"FBbi:00000100"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"}],"CELLTYPE":[{"onto_name":"primary cell line cell","onto_id":"CL:0000001"},{"onto_name":"endothelial cell","onto_id":"CL:0000115"}]}},"Citation":{"Title":"Ana M. Pasapera, Clare M. Waterman (2010) CIL:7440, Homo sapiens, primary cell line cell, endothelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7440","DOI":"doi:10.7295\/W9CIL7440"}}}