Shapiro, Lucy; McAdams, Harley (2021). CIL:40026, Caulobacter crescentus CB15, prokaryotic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07S7NWQ

A maximum intensity projection of a Caulobacter crescentus specimen at 0 degree tilt.

• 2021

• McAdams, Harley
• Shapiro, Lucy

Caulobacteria were induced for 1 hour at 30 degrees C, labeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconverted.

Samples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding, and sectioning at 4µm thickness. A single tilt series of images were acquired using a JEOL 4000, from -60 to 60° at 2° increments, magnification, 40000.0.

Preparation: acrolein fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Data qualification: Processed

• Caulobacter crescentus CB15

• Prokaryotic cell

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40026

Doi: https://doi.org/10.6075/J07S7NWQ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40026

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0HD7VDM

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2022-08-12