{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1341028800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40026.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40026.jpg","Size":54658,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 3700","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=3700"}],"Contributors":["Lucy Shapiro"," Harley McAdams"]},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A maximum intensity projection of a Caulobacter crescentus specimen at 0 degree tilt."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"TECHNICALDETAILS":{"free_text":"Caulobacteria were induced for 1 hour at 30 degrees C, labeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconverted.\n\nSamples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide\/potassium ferrocyanide post-fixation, dehydration and embedding, and sectioning at 4µm thickness.  A single tilt series of images were acquired using a JEOL 4000, from -60 to 60° at 2° increments, magnification, 40000.0."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_id":"FBbi:00000623"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"X"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Caulobacter crescentus CB15","onto_id":"NCBITaxon:190650"},"PREPARATION":[{"onto_name":"acrolein fixed tissue","onto_id":"FBbi:00000005"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"CELLTYPE":{"onto_name":"prokaryotic cell","onto_id":"CL:0000520"}}},"Citation":{"Title":"Lucy Shapiro,  Harley McAdams (2012) CIL:40026, Caulobacter crescentus CB15, prokaryotic cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40026","DOI":"doi:10.7295\/W9CIL40026"}}}