Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:24785, Xenopus laevis, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TH8KFZ

Localization of XAC (Xenopus ADF/cofilin) in Xenopus fibroblasts. Immuno-EM with XAC antibody at high magnification. Low magnification view is available at CIL 24784. Nucleus and surrounding regions look bright because of high content of cellular proteins, which creates high electron density (brightness in inverted contrast). Immunogold-labeling in these central regions is very low. Image corresponds to Figure 10i from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: permeabilized tissue; glutaraldehyde fixed tissue; polyethylene glycol
Relation to intact cell: dispersed cell in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; primary antibody plus labeled secondary antibody; platinum replica; immuno gold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Xenopus laevis

• Actin cytoskeleton
• Fibroblast
• Lamellipodium
• Xenopus ADF/cofilin

• Actin filament-based process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24785

Doi: https://doi.org/10.6075/J0TH8KFZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24785

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12