{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307073600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"24785.tif","Size":14900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"24785.jpg","Size":2732504,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"24785.zip","Size":14814362,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1999 May 31;145(5):1009-26."],"Contributors":["Tatyana M. Svitkina","Gary G. Borisy"],"PUBMED":["10352018"]},"BIOLOGICALPROCESS":{"onto_name":"actin filament-based process","onto_id":"GO:0030029"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Localization of XAC (Xenopus ADF\/cofilin) in Xenopus fibroblasts. Immuno-EM with XAC antibody at high magnification.  Low magnification view is available at CIL 24784.  Nucleus and surrounding regions look bright because of high content of cellular proteins, which creates high electron density (brightness in inverted contrast). Immunogold-labeling in these central regions is very low.  Image corresponds to Figure 10i from J Cell Biol. 1999 May 31;145(5):1009-26."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"free_text":"Xenopus ADF\/cofilin"}],"RELATIONTOINTACTCELL":{"free_text":"dispersed cell  in vitro"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"9875","TECHNICALDETAILS":{"free_text":"Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":2753,"axis":"X"}},{"Space":{"Image_size":1878,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":[{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"polyethylene glycol"}],"VISUALIZATIONMETHODS":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"free_text":"platinum replica"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"free_text":"immuno gold"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Tatyana M. Svitkina, Gary G. Borisy (2011) CIL:24785, Xenopus laevis, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil24785","DOI":"doi:10.7295\/W9CIL24785"}}}