Yam, Patricia T.; Wilson, Cyrus A.; Ji, Lin; Hebert, Benedict; Barnhar, Erin L.; Dye, Natalie A.; Wiseman, Paul W.; Danuser, Gaudenz; Theriot, Julie (2021). CIL:12016, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H41Q5S

Local application of calyculin A (phosphatase inhibitor) can stimulate motility initiation. Phase-contrast time-lapse video of a stationary keratocyte exposed to a local application of calyculin A. Red pseudocolor indicates the calyculin A flow from the micropipette (positioned in the bottom left corner). The keratocyte polarized and initiated motility away from the source of calyculin A. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Corresponds to Fig. 6 C and video 7 in JCB 178:1207-1221, 2007.

• 2021

• Barnhar, Erin L.
• Danuser, Gaudenz
• Dye, Natalie A.
• Hebert, Benedict
• Ji, Lin
• Theriot, Julie
• Wilson, Cyrus A.
• Wiseman, Paul W.
• Yam, Patricia T.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 3422

• Hypsophrys nicaraguensis

• Actin cytoskeleton
• Keratocyte

• Negative regulation of phosphatase activity

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 12016

Doi: https://doi.org/10.6075/J0H41Q5S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12016

Reference

• JCB 178:1207-1221, 2007 https://www.ncbi.nlm.nih.gov/pubmed/?term=17893245
• Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://doi.org/10.1083/jcb.200706012

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12