{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1294894800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"12016.jpg","Size":35606,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"12016_web.mp4","Size":426609,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"12016.zip","Size":2912384,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":[" JCB 178:1207-1221, 2007"],"Contributors":["Patricia T. Yam","Cyrus A. Wilson","Lin Ji","Benedict Hebert","Erin L. Barnhar","Natalie A. Dye","Paul W. Wiseman","Gaudenz Danuser","Julie Theriot"],"PUBMED":["17893245"]},"BIOLOGICALPROCESS":{"onto_name":"negative regulation of phosphatase activity","onto_id":"GO:0010923"},"PROCESSINGHISTORY":{"free_text":"contrast, brightness, image combine"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Local application of calyculin A (phosphatase inhibitor) can stimulate motility initiation. Phase-contrast time-lapse video of a stationary keratocyte exposed to a local application of calyculin A. Red pseudocolor indicates the calyculin A flow from the micropipette (positioned in the bottom left corner). The keratocyte polarized and initiated motility away from the source of calyculin A. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames\/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Corresponds to Fig. 6 C and video 7 in  JCB 178:1207-1221, 2007."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"3422","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":384,"Pixel_size":{"unit":"microns","value":0.549},"axis":"X"}},{"Space":{"Image_size":384,"Pixel_size":{"unit":"microns","value":0.549},"axis":"Y"}},{"Time":{"unit":"seconds","value":5}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Hypsophrys nicaraguensis","onto_id":"NCBITaxon:131240"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"keratocyte"}}},"Citation":{"Title":"Patricia T. Yam, Cyrus A. Wilson, Lin Ji, Benedict Hebert, Erin L. Barnhar, Natalie A. Dye, Paul W. Wiseman, Gaudenz Danuser, Julie Theriot (2011) CIL:12016, Hypsophrys nicaraguensis, keratocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12016","DOI":"doi:10.7295\/W9CIL12016"}}}