Ball, David; Peccoud, Jean (2021). CIL:39597, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FX79JB

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged WHI5. WHI5 is a repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1.These phase,gfp z-series images are part of an image group that ranges from CIL:39596-39600. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

• 2021

• Ball, David
• Peccoud, Jean

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy; phase contrast microscopy
Parameter imaged: fluorescence emission; elastic scattering of photons
Source of contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization methods: EGFP; visualization of contiguous regions

• Cell Image Library Group ID: 10637

• Saccharomyces cerevisiae

• Cytoplasm
• Nucleus
• WHI5 protein

• Cell cycle
• Regulation of cell size
• Regulation of transcription involved in G1/S phase of mitotic cell cycle
• Regulation of transcription, DNA-dependent

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39597

Doi: https://doi.org/10.6075/J0FX79JB

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39597

Reference

• Ball DA, Marchand J, Poulet M, Baumann WT, Chen KC, Tyson JJ, Peccoud J. Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry. PLoS One. 2011;6(10):e26272. https://doi.org/10.1371/journal.pone.0026272
• PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26. https://www.ncbi.nlm.nih.gov/pubmed/?term=22046265

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2022-08-12